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The entire glass membrane must always be clean. Rinsing the membrane with distilled water will often suffice for aqueous solutions. Rinsing the electrode with a mild detergent solution once a week will be beneficial. An alkaline hypochlorite solution can be used to clean electrode membranes subjected to solutions containing fat or proteins.
- High temperature measurements, compounded by constant use in strong alkaline solutions or weak solutions of hydrofluoric acid will drastically reduce the lifetime of the electrode, since the glass membrane will slowly dissolve.
- Dry storage is recommended if the electrode will not be used for two weeks or more. Before use, the electrode should be soaked well. After overnight soaking, rinsing, soaking in a buffer of pH = 4, and again rinsing, the electrode should be ready for use. If a shorter soaking time is necessary, the electrode should be calibrated frequently to adjust for drifting potentials.
- Trapped air bubbles around the inner reference electrode will produce an unstable reading. Swing the electrode in an arc or tap it gently to remove the bubbles. The electrode may have to be heated gently to approximately 60° C in a water bath if the air bubbles are trapped by KCl solution.
- In order to establish a stable, hydrated glass membrane, new or dry-stored electrodes should be soaked overnight in 0.1 M HCl.
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- Be sure to use distilled or deionized water when performing a calibration.
- If the electrode fails to respond as expected, repeat the calibration.
- If the electrode still fails to respond as expected, substitute another electrode that is known to be in good working order for the questionable electrode. If the problem persists and you are using an electrode pair, try the same routine with a working reference electrode.
- If the problem persists, the standards may be of poor quality. Open fresh standards and attempt to calibrate the electrode again.
- If another electrode is not available for test purposes, or if the electrode in use is suspect, review the calibration procedure and be sure to:
- Clean and rinse the electrode thoroughly.
- Prepare the electrode properly.
- Use the proper filling solution.
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